Guilford Techno Consultants, Inc. - Dr. Kimberly A. Conlon

Dr. Kimberly A. Conlon
Guilford Techno Consultants, Inc.
P.O. Box 417
Stony Brook, NY 11790
Phone: (631) 553-5284
E-mail: kconlon@bccmicro.com

Specialties

Organic Chemistry

Extraction and purification of biologically active molecules (natural products)
from higher plants, herbs, seeds and animal sources
Organic synthesis, purification and analysis of product
Chemical modification of biological macromolecules (proteins and nucleic acids)
Photosynthetic reactions, site-directed photocleavage
reactions (photoproteases and photonucleases)

Analytical Chemistry

Chromatographic separations and HPLC
LC/MS/MS
LC/MS
GC/MS
UV-VIS spectroscopy
IR spectroscopy
Mass Spectrometry (MS)
Nuclear Magnetic Resonance Spectrometry (NMR)

Biochemistry

Vector cloning, expression and purification of bacterially-expressed proteins
Protein and nucleic acid biochemistry, enzymatic assays, chemical
modification of proteins and nucleic acids (protein-protein crosslinking,
protein-DNA crosslinking, fluorescent labeling of proteins,
chemical tagging of proteins and nucleic acids)
Production and screening of polyclonal and monoclonal antibodies,
purification of immunoglobulins, IgG isotyping
Immunoassays (Enzyme linked immunosorbent assays (ELISA) (various formats),
immunoblotting (Western blots), immunoprecipitations, affinity columns)

Cell Biology

Bacterial and yeast cell culture, insect and mammalian tissue culture
Immunocytochemistry (immunofluorescence , immunoperoxidase)
Wide-field light microscopy (phase contrast, dark field, epifluorescence, micrography)

Invention Disclosures

Multi-Well Apparatus for Probing Ligands Immobilized Onto Membranes. Invention R-7182 (1996) Berrios, M. and Conlon, K.A. Sponsor: The Research Foundation of the State University of New York

Monoclonal Antibodies Directed Against the Oxidative DNA Lesion 8-Oxoguanine (8-OxoG). Invention R-7224 (1997) Berrios, M. and Conlon, K.A. Sponsor: The Research Foundation of the State University of New York

Method and Compounds for Visible LightInduced SiteDirected Photocleavage of Proteins. Invention R7391 (1999) Conlon, K.A. and Berrios, M. Sponsor: The Research Foundation of the State University of New York

Monoclonal Antibodies Directed Against the Enzyme 8-Oxoguanine DNA Glycosylase. Invention R-8277 (2010) Berrios, M. and Conlon, K.A. Sponsor: The Research Foundation of the State University of New York

Publications

Chapter Contributions

Berrios, M., Conlon, K.A., and Colflesh, D.E. (1999) Antifading Agents for Confocal Fluorescence Microscopy in: Methods in Enzymology. Conn, P. M. (editor). Academic Press, New York. Vol 307, pp. 55-79.

Wachter-Jurcsak, N., Conlon, K.A. (2000) Synthetic Applications of Dienes and Polyenes, Excluding Cycloadditions in: Chemistry of Dienes and Polyenes. Rapopport, Z. (editor). John Wiley & Sons, Chichester, UK. Vol 2, pp. 693-738.

Berrios, M., Conlon, K.A., Colflesh, D.E. (2010) Antifading Agents for Confocal Fluorescence Microscopy in: Reliable Lab Solutions: Techniques in Confocal Microscopy. Conn, P. M. (editor). Elsevier, New York. pp. 47-72.

Peer Reviewed Journals

Sutherland, B.M., Bennett, P.V., Conlon, K.A., Epling, G.A. Sutherland, J.C. (1992) Quantitation of Supercoiled DNA Cleavage in Nonradioactive DNA: Application to Ionizing Radiation and Synthetic Endonuclease Cleavage. Analytical Biochem. 201, 80-6.

Sutherland, B.M., Randesi, M., Wang, K., Conlon, K.A., Epling, G.A. (1993) Promoter-Specific Synthetic Photoendonuclease: Rose Bengal-labeled T7 RNA Polymerase. Biochemistry 32, 1788-1794.

Conlon, K.A., and Berrios, M. (1998) Enzyme-Linked Immunosorbent Assay for Detecting Antigens with Low Binding Affinity to Conventional Microtiter Plates. Analytical Biochem. 263, 246-248.

Colflesh, D.E., Conlon, K.A., and Berrios, M. (1999) Subnuclear Distribution of Drosophila Chromatin Remodeling Protein 1 (CRP1). J. Histotech. 22, 23-30.

Conlon, K.A., Berrios, M. (1999) Immunogen Constructs: A Method for Direct Crosslinking of DNA Bases Containing Aromatic Amino Groups to Proteins. J. Biochem. Biophys. Methods 38, 181-189.

Conlon, K.A., Grollman, A.P., and Berrios, M. (2000) Immunolocalization of 8-Oxoguanine in Nutrient-Deprived Mammalian Tissue Culture Cells. J. Histotech. 23, 37-44.

Conlon, K.A., and Berrios, M. (2001) Light-Induced Proteolysis of Myosin Heavy Chain by Rose Bengal-Conjugated Antibody Complexes. J. Photochem. Photobiol. B, Biology 65, 22-28.

Conlon, K.A., Rosenquist, T., and Berrios, M. (2002) Site-Directed Photochemical Disruption of the Actin Cytoskeleton by Actin-Binding Rose Bengal-Conjugates. J. Photochem. Photobiol. B, Biology 68, 140-146.

Conlon, K.A., Zharkov, D.O., and Berrios, M. (2003) Immunofluorescent Localization of the Murine 8 Oxoguanine DNA Glycosylase (mOGGI) in Cells Growing Under Normal and Nutrient Deprivation Conditions. DNA Repair 2, 1337-1352.

Conlon, K.A., Zharkov, D.O., and Berrios, M. (2004) Cell Cycle Regulation of the Murine 8-Oxoguanine DNA Glycosidase (mOGG1): mOGG1 Associates with Microtubules During Interphase and Mitosis. DNA Repair 3, 1601-1615.

Conlon, K.A., Miller, H., Rosenquist, T., Zharkov, D.O., and Berrios, M. (2005) DNA Polymerase alpha (pol alpha) and NEIL2 Bind Microtubules In Situ and In Vitro. DNA Repair 4, 419-431.

Kemeleva, E.A., Sinitsyna, O.I., Kolosova, N.G., Vasyunina, E.A., Zharkov, D.O., Conlon, K.A., Berrios, M.; Nevinsky, G.A. (2006) Immunofluorescent Detection of 8-Oxoguanine DNA Lesions in Liver Cells from Aging OXYS Rats, a Strain Prone to Overproduction of Free Radicals. Mutation Research 599(1-2), 88-97.

Kemeleva, E.A., Sinitsyna, O.I., Conlon, K.A., Berrios, M., Kolosova, N.G., Zharkov, D.O., Vasyunina, E.A., Nevinsky, G.A. (2006) Oxidation of Guanine in Liver and Lung DNA of Prematurely Aging OXYS Rats. Biochemistry (Moscow) 71(6), 612-618.

Conlon, K.A. and Berrios, M. (2007) Site-Directed Photoproteolysis of 8-Oxoguanine DNA Glycosylase 1 (OGG1) by Specific Porphyrin-Protein Probe Conjugates: A Strategy to Improve the Effectiveness of Photodynamic Therapy for Cancer. J. Photochem. Photobiol. B, Biology 87, 9-17.

Poster Presentations

Epling, G.A., Yang, D-H., Conlon, K.A. (1996) Photosensitized Deprotection of Methylthiomethyl Ethers Using Visible Light. The American Chemical Society 212th National Meeting, Orlando, FL

Conlon, K.A., Grollman, A.P., Berrios, M. (1998) Anti-8-Oxoguanine Antibodies Reveal that Relatively Mild Cellular Stress Induces Oxidative DNA Damage. The American Association for Cancer Research, Annual Meeting, New Orleans, LA

Conlon, K.A., Berrios, M. (2001) Antibody-Directed Photolysis of Skeletal Myosin Heavy Chain. The American Society for Cell Biology 41st Annual Meeting, Washington, DC

Conlon, K.A., Rosenquist, T., Berrios, M. (2002) Site-Directed Photochemical Disruption of the Cytoskeleton by Actin-Binding Rose Bengal-Conjugates. The American Society for Cell Biology 42nd Annual Meeting, San Francisco, CA

Conlon, K.A., Berrios, M., Zharkov, D.O. (2003) Subcellular Distribution of mOGG1 in Response to Oxidative Stress. The American Society for Cell Biology 43rd Annual Meeting, San Francisco, CA

Conlon, K.A., Zharkov, D.O., Berrios, M. (2004) 8-Oxoguanine DNA Glycosylase Associates with Microtubules During Interphase and Mitosis. The American Society for Cell Biology 44th Annual Meeting, Washington, DC

Conlon, K.A., Rosenquist, T.A., Sidorenko, V., Iden, C.R. (2010) Quantitation of Aristolochic Acid Adducts in Murine DNA. The American Chemical Society 240th National Meeting, Boston, MA

Conlon, K.A., Dickman, K.G., Romanov, V.I., Iden, C.R. (2011) LC/MS/MS Quantitation of Citric Acid Cycle (CAC) Intermediates in Response to Aristolochic Acid Exposure. The American Society for Mass Spectrometry 59th Annual Conference on Mass Spectrometry and Allied Topics, Denver, CO